serotonin transporter sert (Alomone Labs)
Structured Review
Serotonin Transporter Sert, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serotonin+transporter+sert/Anti-Serotonin+Transporter+Antibody/pm41824184-71-77-79
Average 93 stars, based on 12 article reviews
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Incubation:Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson’s disease by modulating butyrate-producing gut bacteria Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by Mini-PROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson's disease by modulating butyrate-producing gut bacteria. Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by MiniPROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): a) Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), Blocking Assay:Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson’s disease by modulating butyrate-producing gut bacteria Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by Mini-PROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson's disease by modulating butyrate-producing gut bacteria. Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by MiniPROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): a) Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), |
