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serotonin transporter sert  (Alomone Labs)


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    Structured Review

    Alomone Labs serotonin transporter sert
    Serotonin Transporter Sert, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/serotonin+transporter+sert/Anti-Serotonin+Transporter+Antibody/pm41824184-71-77-79
    Average 93 stars, based on 12 article reviews
    serotonin transporter sert - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson’s disease by modulating butyrate-producing gut bacteria
    Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by Mini-PROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), serotonin transporter-SERT (Alomone labs AMT004, 1:200), GPR109 (Invitrogen PA5-90,579, 1:500). .. Colon: ocludin (Santa cruz sc-133256, 1:500); zonula occludens (Santa cruz—sc-33725, 1:500); phospho-α synuclein (Abcam ab51253, 1:500); phospho-NFkB (Cell signalling #3003, 1:1000); IL-1β (abcan ab9722, 1:1000).

    Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson's disease by modulating butyrate-producing gut bacteria.
    Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by MiniPROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): a) Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), serotonin transporter-SERT (Alomone labs AMT004, 1:200), GPR109 (Invitrogen PA590,579, 1:500). b) Colon: ocludin (Santa cruz sc-133256, 1:500); zonula occludens (Santa cruz—sc-33725, 1:500); phospho-α synuclein (Abcam ab51253, 1:500); phospho-NFkB (Cell signalling #3003, 1:1000); IL-1β (abcan ab9722, 1:1000). .. After washing, the membranes were incubated with peroxidase-conjugated anti-rabbit horseradish (HRP) (Sigma-Aldrich A9169, 1:8000), and anti-mouse HRP (Sigma-Aldrich A0161, 1:5000).

    Blocking Assay:

    Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson’s disease by modulating butyrate-producing gut bacteria
    Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by Mini-PROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), serotonin transporter-SERT (Alomone labs AMT004, 1:200), GPR109 (Invitrogen PA5-90,579, 1:500). .. Colon: ocludin (Santa cruz sc-133256, 1:500); zonula occludens (Santa cruz—sc-33725, 1:500); phospho-α synuclein (Abcam ab51253, 1:500); phospho-NFkB (Cell signalling #3003, 1:1000); IL-1β (abcan ab9722, 1:1000).

    Article Title: Prebiotics attenuate depressive-like behavior, neuroinflammation and synaptic plasticity in Parkinson's disease by modulating butyrate-producing gut bacteria.
    Article Snippet: The proteins (30 μg/μL) were separated on sodium dodecyl sulfate (SDS) polyacrylamide by gel electrophoresis under reduced conditions and were then transferred to the nitrocellulose membrane (THERMO SCIENTIFIC 88018) by MiniPROTEAN® precast gels (BIO-RAD 1658004). .. After, the membranes were blocked with 3% BSA and incubated overnight with the following antibodies diluted in blocking solution (1.5% BSA, 0.02% Tris phosphate-buffered, and 0.01% Tween): a) Substantia nigra and pre-frontal cortex: tyrosine hydroxylase (Merck AB152, 1:500), α-synuclein (Santa Cruz, 1:1000), IBA-1 (Wako 016-200001, 1:500), phospho-α synuclein (Abcam, ab51253), anti IL-1β (Genway GWP-BPB232, 1:1000), anti-p-NFkB (Abcam, ab97726, 1:1000), p-CREB (Cell signaling #9198, 1:1000), anti-iNOS (BD 610600, 1:1000) and BDNF (Alomone ANT-10, 1,500), PSD-95 (Alomone #APZ-009, 1:500), serotonin transporter-SERT (Alomone labs AMT004, 1:200), GPR109 (Invitrogen PA590,579, 1:500). b) Colon: ocludin (Santa cruz sc-133256, 1:500); zonula occludens (Santa cruz—sc-33725, 1:500); phospho-α synuclein (Abcam ab51253, 1:500); phospho-NFkB (Cell signalling #3003, 1:1000); IL-1β (abcan ab9722, 1:1000). .. After washing, the membranes were incubated with peroxidase-conjugated anti-rabbit horseradish (HRP) (Sigma-Aldrich A9169, 1:8000), and anti-mouse HRP (Sigma-Aldrich A0161, 1:5000).



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    Co‐expression of Rho‐kinases 1 and 2 with <t>SERT</t> or DAT. A: Representative immunoreactivity images of Rho‐kinase 1 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). B: Percentage of Rho‐kinase 1 and the SERT or DAT double‐positive area to the SERT or DAT‐positive area in the NAc. C: Representative immunoreactivity images of Rho‐kinase 2 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). D: Percentage of Rho‐kinase 2 and SERT or DAT double‐positive area relative to the SERT or DAT‐positive area in the NAc. Data represent the mean + SEM ( n = 5). DAT, dopamine <t>transporter;</t> SERT, serotonin transporter; NAc, nucleus accumbens.
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    Co‐expression of Rho‐kinases 1 and 2 with <t>SERT</t> or DAT. A: Representative immunoreactivity images of Rho‐kinase 1 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). B: Percentage of Rho‐kinase 1 and the SERT or DAT double‐positive area to the SERT or DAT‐positive area in the NAc. C: Representative immunoreactivity images of Rho‐kinase 2 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). D: Percentage of Rho‐kinase 2 and SERT or DAT double‐positive area relative to the SERT or DAT‐positive area in the NAc. Data represent the mean + SEM ( n = 5). DAT, dopamine <t>transporter;</t> SERT, serotonin transporter; NAc, nucleus accumbens.
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    Co‐expression of Rho‐kinases 1 and 2 with <t>SERT</t> or DAT. A: Representative immunoreactivity images of Rho‐kinase 1 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). B: Percentage of Rho‐kinase 1 and the SERT or DAT double‐positive area to the SERT or DAT‐positive area in the NAc. C: Representative immunoreactivity images of Rho‐kinase 2 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). D: Percentage of Rho‐kinase 2 and SERT or DAT double‐positive area relative to the SERT or DAT‐positive area in the NAc. Data represent the mean + SEM ( n = 5). DAT, dopamine <t>transporter;</t> SERT, serotonin transporter; NAc, nucleus accumbens.
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    Co‐expression of Rho‐kinases 1 and 2 with <t>SERT</t> or DAT. A: Representative immunoreactivity images of Rho‐kinase 1 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). B: Percentage of Rho‐kinase 1 and the SERT or DAT double‐positive area to the SERT or DAT‐positive area in the NAc. C: Representative immunoreactivity images of Rho‐kinase 2 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). D: Percentage of Rho‐kinase 2 and SERT or DAT double‐positive area relative to the SERT or DAT‐positive area in the NAc. Data represent the mean + SEM ( n = 5). DAT, dopamine <t>transporter;</t> SERT, serotonin transporter; NAc, nucleus accumbens.
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    Image Search Results


    Co‐expression of Rho‐kinases 1 and 2 with SERT or DAT. A: Representative immunoreactivity images of Rho‐kinase 1 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). B: Percentage of Rho‐kinase 1 and the SERT or DAT double‐positive area to the SERT or DAT‐positive area in the NAc. C: Representative immunoreactivity images of Rho‐kinase 2 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). D: Percentage of Rho‐kinase 2 and SERT or DAT double‐positive area relative to the SERT or DAT‐positive area in the NAc. Data represent the mean + SEM ( n = 5). DAT, dopamine transporter; SERT, serotonin transporter; NAc, nucleus accumbens.

    Journal: Neuropsychopharmacology Reports

    Article Title: Distinct Effects of Nonselective Rho‐Kinase Inhibitor Fasudil and Selective Rho‐Kinase 2 Inhibitor KD025 on Serotonin and Dopamine Release in the Nucleus Accumbens of Mice

    doi: 10.1002/npr2.70124

    Figure Lengend Snippet: Co‐expression of Rho‐kinases 1 and 2 with SERT or DAT. A: Representative immunoreactivity images of Rho‐kinase 1 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). B: Percentage of Rho‐kinase 1 and the SERT or DAT double‐positive area to the SERT or DAT‐positive area in the NAc. C: Representative immunoreactivity images of Rho‐kinase 2 (green), SERT (magenta), DAT (red), and Hoechst 33342 (blue) in the NAc (scale bar indicates 10 μm). D: Percentage of Rho‐kinase 2 and SERT or DAT double‐positive area relative to the SERT or DAT‐positive area in the NAc. Data represent the mean + SEM ( n = 5). DAT, dopamine transporter; SERT, serotonin transporter; NAc, nucleus accumbens.

    Article Snippet: The following primary antibodies were used: anti‐Rho‐kinase 1 (Cat#21850–1‐AP, RRID:AB_10953526, 1:100 dilution; Proteintech, IL, USA), anti‐Rho‐kinase 2 (Cat# Ab125025 , RRID:AB_10972853, 1:200 dilution; Abcam, Cambridge, UK), anti‐serotonin transporter (SERT) (Cat#MSFR103270, 1:200 dilution; Nittobo Medical, Tokyo, Japan), and anti‐dopamine transporter (DAT) (Cat#sc‐32258, RRID:AB_627400, 1:250 dilution, Santa Cruz Biotechnology, Texas, USA).

    Techniques: Expressing